olsen hegar needle holder  (Fine Science Tools)

Journal: STAR Protocols

Article Title: Population imaging of synaptically released glutamate in mouse hippocampal slices

doi: 10.1016/j.xpro.2021.100877

Figure Lengend Snippet:

Article Snippet: Olsen-Hegar Needle Holders with Suture Cutters , Fine Science Tools , Cat#12502-12.

Techniques: Virus, Plasmid Preparation, Recombinant, Software, Injection, Transferring, Dissection, Microscopy, Adhesive, Fluorescence, Light Microscopy, Isolation

Journal: Journal of visualized experiments : JoVE

Article Title: Establishing in situ closed circuit perfusion of lower abdominal organs and hind limbs in mice

doi: 10.3791/60847

Figure Lengend Snippet: Equipment and Materials

Article Snippet: ​ table ft1 table-wrap mode="anchored" t5 caption a7 Name of Material/ Equipment Company Catalog Number Comments/Description Equipment SCP plugsys servo control F/Perfusion (part 1 in ) Harvard Apparatus 732806 control the purfusion speed Thermocirculator (part 2 in ) Harvard Apparatus 724927 circulating water bath for all water-jacketed components Moist chamber cover with quartz window (part 3 in ) Harvard Apparatus 733524 keep the chamber’s temperature Roller pump (part 4 in ) Harvard Apparatus 730113 deliver perfusate to cannula in the moist chamber Three-way stopcock (part 5 in ) Cole-Palmer 30600-02 Moist chamber bubble trap (part 6 in ) Harvard Apparatus 733692 Customized as the perfisate container; also enabled constant pressure perfusion Silicone tubing set (arrows in ) Harvard Apparatus (TYGON) 733456 Moist chamber with metal tube heat exchanger Harvard Apparatus 732901 Water-jacketed moist chamber with lid to maintain perfusate and mouse temperature Silicone pad Harvard Apparatus Table for moist chamber Harvard Apparatus 734198 Veterinary anesthesia machine Highland HME109 Carbon dioxide, USP Airgas healthcare 19087-5283 Oxygen compressed, USP Airgas healthcare C2649150AE06 3.5X-90X stereo zoom microscope on boom stand with LED light Amscope SKU: SM-3BZ-80S Confocal microscope NIKON ECLIPSE Ti2 Disposable Sterile Cautery Pen with High Temp FIAB F7244 Olsen-Hegar needle holders with suture cutters Fine Science Tools (FST) 125014 Student standard pattern forceps Fine Science Tools (FST) 91100-12 Surgical Scissors Fine Science Tools (FST) 14001-14 Materials Permanent marker Staedtler 342-9 Filter (0.2 μm) ThermoFisher 42225-CA Filter for 5% BSA-RINGER’S Syringe (60ml) BD 309653 Filter for 5% BSA-RINGER’S Syringe (10 ml) Fisher Scientific 14-823-2E 19-G BD PrecisionGlide needle BD 305186 For immobilizing the Insyte Autoguard Winged needle and scratching the cortical bone 4-0 silk sutures Keebomed-Hopemedical 427411 6-0 silk sutures Keebomed-Hopemedical 427401 Reagents Isoflurane Piramal Enterprises Limited 66794-017-25 Ethanol (70% ( vol/vol )) Pharmco 111000190 Baxter Viaflex lactate Ringer’s EMRN Medical Supplies Inc. JB2324 Bovine serum albumin Thermo Fisher 11021-037 Hoechst33342 Life Technologies H3570 DyLight-649-lectin Vector Laboratories,Inc.

Techniques: Microscopy, Marker, Plasmid Preparation, Mouse Assay